To distribute the spores equally in the substrate, shake the bag carefully.įor colonisation of the substrate, store the bag at the convenient 'spawn run' - temperature (see product descriptions) in a dark place. Seal the inoculation area and distribute the sporesĬlose the inoculation area immediately with the tape. After that let the needle cool off for a few seconds (do not overheat the needle as the stand is made of plastic which could melt!). The front part of the needle should glow red. Heat the needle using an alcohol lamp (or a lighter). Should the needle get in contact with any unsterile material, you will have to heat-sterilize it. For larger bags (4,5 L content) we recommend to use 20 - 30 ml spore solution – that is 2 - 3 spore syringes. We recommend to use about 10 - 20 ml – that is 1 - 2 syringes - for small bags (2,5 L content).
Now put the needle into the bag (at the disinfected area, always above substrate level) and push the spore solution of the same species into the bag. Shake the syringe to dispense the spores equally. Kindly spray disinfection (e.g., Bacillol) on the bag, let it react for about 20-30 seconds and then dry it with a clean kitchen paper or similar. To avoid invasion of any contamination during inoculation, we recommend to disinfect the surface (the place you want to put the needle in). Substrate bag (depending to the mushroom species) – rye-Vermiculite mix with max. As soon as the substrate is fully colonised by the mushroom, you can set up the bag for fruiting. When you bring spores onto an appropriate nutrient medium, the spores start to germinate. Detailed information about the convenient substrate can be found in the product description of our spores. Use a convenient substrate, depending on the mushroom species you want to cultivate. This is a very simple method of mushroom cultivation and suitable for hobby-mycologists. Inoculation of substrate bags with spore syringes King stropharia - Stropharia rugosoannulata.Brown-Gilled woodlover - Hypholoma capnoides.Umbrella Polypore - Polyporus Umbellatus.Caterpillar fungus - Cordyceps sinensis.Winter mushroom (Enokitake) - Flammulina velutipes.Almond mushroom - Agaricus blazei murril.Beech mushroom - Hypsizygus tessellatus.Golden Oyster - Pleurotus citrinopileatus.Pink Oyster Mushroom - Pleurotus salmoneostramineus.
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